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What is the working principle of FXG series fermentation tanks
source：www.sjbyj.com Release time：2026-09-08
The FXG series belongs to the vertical mechanical stirring liquid deep fermentation tank, which is mainly used for microbial aerobic/anaerobic fermentation in the fields of pharmaceuticals, biological products, food, and chemical engineering. The contact material parts are made of sanitary grade stainless steel, and the overall meets GMP specifications. The core principle of the equipment is to build a closed and sterile environment, and through the collaboration of multiple systems, regulate the temperature, dissolved oxygen, and other factors required for microbial growth pH、 Under conditions such as tank pressure, the bacterial strain utilizes the nutrients in the culture medium for proliferation and metabolism, producing the target product.
The entire process is divided into four stages: sterilization, inoculation, fermentation and cultivation, and discharge.
1. Sterilization stage (SIP in-situ sterilization)
Inject saturated steam into the jacket of the tank body to sterilize the internal cavity, culture medium, and auxiliary pipeline valves of the tank body at high temperature, killing impurities and spores inside the tank body; After sterilization is completed, the jacket switches to cooling water to cool the materials inside the tank to the process set inoculation temperature, establishing a sterile reaction environment. There are no hygiene dead corners in the tank and pipelines, and a sterile respiratory system is used to prevent external bacteria from entering the tank.
2. Internal circulation stirring dissolved oxygen transfer principle (core structural principle)
The motor drives the rotation of multiple layers of stirring blades inside the tank, forming a circulating flow of liquid inside the tank. Inject sterile filtered compressed air into the bottom, and the gas enters the liquid phase to form bubbles; Stirring blades shear and crush bubbles, breaking large bubbles into small ones, increasing the gas-liquid contact area, and improving the efficiency of oxygen dissolution into the fermentation broth.
Aerobic fermentation: relying on the linkage of stirring speed and ventilation to adjust dissolved oxygen DO;
Stir while fully mixing the bacterial cells and nutrients, eliminating local concentration and temperature gradients in the tank;
The top of the tank is equipped with a demister to prevent foam generated by fermentation metabolism and avoid contamination of the sterile system by escaping liquid.
3. Temperature control system
The tank is equipped with a jacket (some large-sized tanks are equipped with inner coil tubes), which achieves heating and cooling through jacket medium heat exchange. Introduce steam or hot water when heating is required; Need to cool down and introduce cooling water; The PT100 temperature probe collects real-time temperature inside the tank, and the PLC closed-loop automatically adjusts the medium flow rate to maintain a stable temperature range suitable for microbial growth.
shtu、 Supplement materials and maintain a sterile environment for tank pressure
PH online electrodes are installed inside the tank. Microbial metabolism during fermentation can cause pH deviation, and the system automatically adds acid and alkali solutions to adjust the acidity and alkalinity.
A sterile respiratory system maintains a slight positive pressure inside the tank, which can prevent external bacteria from invading; The exhaust gas is discharged after passing through the sterilization filter; Feeding can be carried out according to the process, by adding nutrients such as carbon and nitrogen sources through sterile feeding ports.
5. Online monitoring and process control
Real time temperature collection by online sensors pH、 Dissolved oxygen DO, tank pressure, stirring speed; The control system adjusts the stirring speed, intake flow rate, jacket cold and hot media, acid-base replenishment, and defoaming spray in conjunction with the process settings. When the fermentation reaches the set endpoint indicators such as time and product concentration, stop stirring and ventilation, and complete the fermentation liquid discharge from the bottom outlet.
6. Adaptation to anaerobic conditions
If anaerobic fermentation is carried out, close the sterile air intake and replace the oxygen in the tank; Relying on a sealed positive pressure sterile respiratory system to isolate air, stirring is only used for material mixing and no longer responsible for dissolved oxygen function.
The entire process is divided into four stages: sterilization, inoculation, fermentation and cultivation, and discharge.
1. Sterilization stage (SIP in-situ sterilization)
Inject saturated steam into the jacket of the tank body to sterilize the internal cavity, culture medium, and auxiliary pipeline valves of the tank body at high temperature, killing impurities and spores inside the tank body; After sterilization is completed, the jacket switches to cooling water to cool the materials inside the tank to the process set inoculation temperature, establishing a sterile reaction environment. There are no hygiene dead corners in the tank and pipelines, and a sterile respiratory system is used to prevent external bacteria from entering the tank.
2. Internal circulation stirring dissolved oxygen transfer principle (core structural principle)
The motor drives the rotation of multiple layers of stirring blades inside the tank, forming a circulating flow of liquid inside the tank. Inject sterile filtered compressed air into the bottom, and the gas enters the liquid phase to form bubbles; Stirring blades shear and crush bubbles, breaking large bubbles into small ones, increasing the gas-liquid contact area, and improving the efficiency of oxygen dissolution into the fermentation broth.
Aerobic fermentation: relying on the linkage of stirring speed and ventilation to adjust dissolved oxygen DO;
Stir while fully mixing the bacterial cells and nutrients, eliminating local concentration and temperature gradients in the tank;
The top of the tank is equipped with a demister to prevent foam generated by fermentation metabolism and avoid contamination of the sterile system by escaping liquid.
3. Temperature control system
The tank is equipped with a jacket (some large-sized tanks are equipped with inner coil tubes), which achieves heating and cooling through jacket medium heat exchange. Introduce steam or hot water when heating is required; Need to cool down and introduce cooling water; The PT100 temperature probe collects real-time temperature inside the tank, and the PLC closed-loop automatically adjusts the medium flow rate to maintain a stable temperature range suitable for microbial growth.
shtu、 Supplement materials and maintain a sterile environment for tank pressure
PH online electrodes are installed inside the tank. Microbial metabolism during fermentation can cause pH deviation, and the system automatically adds acid and alkali solutions to adjust the acidity and alkalinity.
A sterile respiratory system maintains a slight positive pressure inside the tank, which can prevent external bacteria from invading; The exhaust gas is discharged after passing through the sterilization filter; Feeding can be carried out according to the process, by adding nutrients such as carbon and nitrogen sources through sterile feeding ports.
5. Online monitoring and process control
Real time temperature collection by online sensors pH、 Dissolved oxygen DO, tank pressure, stirring speed; The control system adjusts the stirring speed, intake flow rate, jacket cold and hot media, acid-base replenishment, and defoaming spray in conjunction with the process settings. When the fermentation reaches the set endpoint indicators such as time and product concentration, stop stirring and ventilation, and complete the fermentation liquid discharge from the bottom outlet.
6. Adaptation to anaerobic conditions
If anaerobic fermentation is carried out, close the sterile air intake and replace the oxygen in the tank; Relying on a sealed positive pressure sterile respiratory system to isolate air, stirring is only used for material mixing and no longer responsible for dissolved oxygen function.
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